Effect Of Ethyl Acetate Extract Of Sappanwood On The Level Of IL-6 In Peripheral Blood Of Cardiac Allograft Rats

Objectives:To observe the effect of ethyl acetate extracted Chinese medicine sappan wood on the levels of cell factor IL-6 in blood of rats with allogenic heart transplantion and the pathomorphology of heterotopic heart muscles,and investigate the immunosuppressive function and possible mechanism.Methods:Choose the improved Ono mode and replicate the allogenic cardiac transplantation model.The model was established by using Wistar rats as donors and SD rats as recipients and the rats with successful operations were divided into three groups, eight rats per group,i.e.model group were given intragastric administration with olive oil depending on rats weight, cyclosporine A group were given CsA according to 35mg/ (kg·d) and sappan wood group were given sappan acetic ether extract depending on 1.15g/(kg·d) . Blood samples were taken on the eleventh postoperation day for the measurement of IL-6 ,and the pathomorphology of heart muscles was observed by routine methods.Results:Ethyl acetate extracted Chinese medicine sappan wood and CsA could obviously reduce the levels of IL-6 in blood of rats,but Sappan wood group inferior to cyclosporine A group.Compared with the model group,the sappan wood and CsA groups of rats showed obvious improvement in the pathologic changes in heart muscles,but Sappan wood group inferior to cyclosporine A group.Conclusions: sappan acetic ether extract improves the pathologic changes in heart muscles and has an obvious effect on anti-immun rejection,which gives rein to immunodepression probably by reducing the secretion of IL-6.