NSAIDs' Influence On Protein Expression Of COX-2, HMLH1 And HMSH2 In BGC-823 Cell Line

Objective: In China, gastric carcinoma is a severe disease that threatens the public health. Previous studies show that the absence or reduction of mismatch repair enzyme (MMR) is closely associated with tumorigenesis. MMR is an important enzyme and plays an important role in the DNA repair system. Its function is to correct the mispaired nucleotides during DNA synthesis. There are nine mismatch repair enzymes in MMR superfamily, of which the most important members are human mut-l homologue 1 (hMLH1) protein and human mut-s homologue 2 (hMSH2). Both are absent or exhibit a low expression in many tumors. When the expression of MMR is absent or reduced, the replication errors of microsatellite (MS) can not be repaired, leading to the microsatellite instability (MSI). MSI means the increase or decrease of simple nucleotide repeat units due to the replication error. MSI can increase the instability of genomic DNA, and the frequency of random mutation, resulting in the changes in a variety of tumor-related genes and followed by the occurrence of tumor.Cyclooxygenase-2 (COX-2) is the rate-limiting enzyme in the process of prostaglandin synthesis. Over the past several years, a large body of evidences have shown that COX-2 protein,...