Hyperhomocysteine, Genetic Polymorphism Of Methylenetetrahydrofolate Reductase In Young Adults With Ischemic Stroke

Objective: To investigate the relationships of polymerphi-sms of methylenetetrahydrofolate reductase (MTHFR) gene and plasma homocysteine(Hcy) levels with ischemic stroke in young Adults .Methods: The plasma Hcy levels and genetic polymer-phism of MTHFR among forty-seven cases with young adult ischemic cerebral disease(37 males, 10 females; mean±SD age, 42.9±6.3 years; age at first event, <50 years) were investigated, 32 age and sex matched healthy persons(21 males, 11 females; mean±SD age, 44.2±5.6 years) and 39 persons with neural system but vascular disease(21 males, 18 females; mean±SD age,43.7±5.9 years) were used as controls. Their polymorphisms of MTHFR gene were analyzed using PCR-RFLP and plasma Hcy levels were measured using fluorescence polarization immunoassay (FPIA). All the subjects underwent brain CT or MRI examination, serum vitaminB₁₂and folic acid examination, besides examination of plasma Hcy and nucleotide 677 MTHFR genotypes. The effects of age, sex, liver function, renal function ,smoking ,drinking , were analyzed.Results:...