Experimental Study Of The Effect Of Ligustrazin Liquid Injection On Rats With FGR-induced By Smoking And Drinking Alcohol

Objective: To study the effect of Ligustrazin liquid injection on rats with FGR-induced by smoking and drinking alcohol from growth parameter growth-faceors NO and morphology, to find out the effect and mechanism of Ligustrazin liquid injection and to provide the important and scientific basis for the new treatment of FGR.Methods: 1. The model of FGR rats was made by means of passive smoking and drinking alcohol. Some pregent rats were treated with Ligustrazin liquid injection.,birth weight, the length and Poundera Index of all fetal rats and the rate of FGR in the three groups were measured and compared.To explore the effect of Ligustrazin on FGR. 2. The seruns levels of IGFs in rats of the three group was determined by method of RIA. 3. Immunohistochemical assay was performed to detect the IGF-â… and IGF-â… R expression on placenta and fetals'liver. To explore the effect of Ligustrazin on the synthesis and excretion of growth-factors of FGR rats. 4. The NO of serum was measured in the shade selection method by spectrophotometer. 5. The placenta of rats were examined by HE-stainsection. The tissue were assessed for morphological change with light microscope and electron microscope.Results: 1. In the experimental group, the FGR rats'body weight, height were significantly lower than those control group(P<0.05). 2. The serums levels of IGFs in fetals of FGR group were lower than those in normal groups(P<0.05), But there was no significant difference in the pregnancied rats of three groups(P>0.05). 3. In the FGR rats'placenta, the expression of IGF-â… was increased and IGF-â… R was decreased(P<0.05), and in the FGR fetals'liver, the expression of IGF-â… was decreased and IGF-â… R was increased(P<0.05), compared with those in the control group. These indexes were reversed after the treatment of Ligustrazin liquid injection, and fetal growth and development in treated group were near to those in the normal control group. 4.Compared with normal group,the seruns levels of NO in the pregnancied rats of FGR group(P<0.05). And it is increased after treating with Ligustrazin liquid injection(P<0.05). 5.The obvious placental morphologic change soccurred in rats with FGR-induced by smoking and drinking alcohol . Ligustrazin liquid injection can release this kind of change.Conclusions: 1.The expression of IGF-â… and IGF-â… R were related to the FGR.The effect...