Nucleic Acid Amplification Tests To Detect Neisseria Gonorrhoeae

Objective: to evaluate and compare culture with two nucleic acid amplification tests (NAATs) for the detection of Neisseria gonorrhoeae. Development of a rapid, sensitive, and accurate assay for confirmation of Neisseria gonorrhoeae in clinical samples.Methods: Two polymerase chain reaction (PCR) assays, developed on the amplification of the N gonorrhoeae cppB gene and 16S rRNA gene, were utilised for detection of this NG in clinical swab samples .Performance characteristics of the two assays were compared with result of NG culture. Two of three reslts are positive which clinical samples be considered as positive.Results: Three motheds have similar specificity,but sensitivity from PCR are higher than culture.the sensitivity from urine detection is very low. 15 clinical samples positive and another 139 samples negative for N gonorrhoeae culture , 24 clinical samples positive and another 129 samples negative for N gonorrhoeae cppB PCR , 27 clinical samples positive and another 127 samples negative for N gonorrhoeae 16s Rrna PCR,Overall, 26 of 154 (16.9%) samples were confirmed as true positive. The two real time assays had sensitivities of 92.3% and 100% and specificities of 100% and 99.2%, indicating a similar sensitivity to and specificity of both assaysConclusion: The data from this study highlight the need to have a better mothod to detect NG. nucleic acid amplification tests overcome many barriers which culture have. The two PCR assays may be new methods for detection of Neisseria gonorrhoeae.