The Infection Of Hepatitis B Virus In Renal Tissues Of 57 Patients With IgA Nephropathy

objective To investigate the existence of hepatitis B virus (HBV) in renal tissues from patients with IgA nephropathy(IgAN),To explore the correlation between hepatitis B virus antigens and the infiltrated leucocytes and the expression of intercellular adhesion molecule-1(ICAM-1).Methods Renal biopsy specimens from 57 patients(1 case with serum hepatitis B virus surface antigen,56 cases with negative serum HBV markers) with primary IgA nephropathy were examined by use of Envision immunohistochemistry (HBsAg,HBcAg,CD3,CD68 and ICAM-1) techniques. 14 fresh renal tissues specimens of the 57 patients were detected HBV DNA by use of fluorescence quantitative polymerase chainreaction (FQ-PCR) method.Results1 HBV DNA was detected in 1 case of IgAN renal tissues with serum hepatitis B virus surface antigen(1/14),the case was also detected renal tissue HBAg(HBsAg,HBcAg). HBAg in renal tissues of the other 56 cases with negative serum HBV markers was observed in 17(30.4%),and HBAg were mostly found in tubular epithelial cells;2 The degree of interstitial cellular infiltration in IgAN with HBAg positive group was significantly more serious compared with negative group.(P<0.05);3 Contrary to the HBAg negative group,The degree of both interstitial infiltrated CD3+ cells and tubular ICAM-1+ stain area of positive group were statistically significantly higher(P<0.01, P<0.01).4 The numbers of the interstitial infiltrated CD68+ cells was not significant between renal HBAg positive group and negative group(P>0.05).Conclusions1 Renal tissues of some IgA nephropathy are directly infected with HBV ,and the cellular mechanism mediated by HBV originating from renal cells in situ may be involved in the pathogenesis of IgA nephropathy;2 The renal tubular epithelial cells may be the targeting cells of HBV infection;3 ICAM-1 and T lymphocytes may play an part in the inflammation of HBV infection.