Sentinel Lymph Nodes Detection In Patients With Cervical Cancer And Its Expression Of CD80 CD83 CD86 In Dendritic Cell

Objective: To investigate the feasibility of sentinel lymph node (SLN) detection in patients with cervical cancer.;To study its expression of CD83 CD80 CD86 in dentritic cell .and investigate the immune states of sentinel lymph node and the mechanisms by which tumors escape immune recognition .Methords: From December 2004 to December 2005,fourty-five patients with cervical cancer at stageâ… a(n=5),â… b1(n==24),â… b2(n==12),â…¡a(n==4) underwent SLN detection by using blue dye (patent blue violet 1%).Four ml of patent blue violet 1% was injected into the cervix at 4 points around the tumor just at the time radical hysterectomy and extensive biolateral pelvic lymphadenectomy.Tumor characteristics and specific locations of lymphatic dye uptake were recorded.The samples of SLN and non-SLN(NSLN) were compared with the expression of CD83 CD80 CD86 in dendritic cell.Result: (1)Out of 45 patients ,SLNS were detected in 40 patients.A total of 86 SLNS were identified and the mean was 2 per patients.The detection rate of SLN was 89%.Six patients(13%) were diagnosed with lymph node metastase and all 6 patients are in the group SLN.One patient had both positive SLNS and pelvic lymph node.No one had positive pelvic lymph node and negative SLN,The predictive rate was 100% and the false -negative rate was zero.(2)The expression rates of CD83,CD80,CD86 inmetastatic SLN were less than those in non-metastatic SLN and non-SLN. There was no significant difference in the rates of CD83,CD80,CD86 between non-metastatic SLN and non-SLN.Conclusion: (1)SLN detection can successfully predict the pelvic lymph node status in patients with early cervical cancer..(2) The metastatism of cervical cancer lymph node is related with the number of dendritic cell of CD83,CD80,CD86 decreases.(3)Patients with early cervical cancer can be avoided with conventional pelvic lymph node excision.in the face of immunology.