The Study On Quality Control Standard And The Anti-fatigue Activity For Health Food ShenHaGen Soft Capsules

Objective: Ginsenoside Rgl and Puerarin were determined by HPLC to establish thequality control standard of ShenHaGen soft capsules, which is a kind of healthy food.Investigate the anti-fatigue activity of ShenHaGen soft capsule elementarily basing onthe method for the Assessment of Alleviating Physical fatigue Function. Methods:The HPLC method to determine Ginsenoside Rg1: after being extracted by SoxhletExtraction and ultrasonic, the sample was separated by Phenomenx C₁₈column(4.6mm×250mm,5μm). The mobile phase was CH₃CN-0.05%H₃PO₄(20.3:79.7, V/V); the flow rate was 1.0 mL·min^-1; the wavelength was 203nm and thecolumn temperature was 30℃. The HPLC method to determine Puerarin: after beingextracted by aether and ultrasonic, the sample was separated by Phenomenx C₁₈column(4.6mm×250mm,5μm). The mobile phase was CH₃OH-0.05%H₃PO₄(25:75,V/V); the flow rate was 0.8 mL·min^-1; the wavelength was 250nm and thecolumn temperature was 35 ℃. Mice were treated orally with different doses ofShenHaGen soft capsule to investigate the anti-fatigue activity. The time of miceswimming with loads, serum urea and lactic acid level were determined after 30minutes from the last administration. Rusult: The linear range and average ofrecovery of Ginsenoside Rgl in ShenHaGen soft capsules was 1.24～6.2μg(r=0.9999)and 101.883%(RSD=1.114%), while those of Puerarin in ShenHaGen soft capsuleswas 0.036～0.432μg(r=0.9999) and 99.688%(RSD=0.186%)respectively. Within thedefinite range of ShenHaGen soft capsules, the period of mice swimming with loadsextended; serum urea decreased; but the effect of the area coverd by blood lactic acidcurve was not significantly changed. Conclusion: The two methods to determineGinsenoside Rgl and Puerarin by HPLC were simple. The results were accurate andreliable. It can be used to control the quality of ShenHaGen soft capsules. The healthfood ShenHaGen soft capsule had an alleviating physical fatigue function to someextent within the definite range, but the range was narrow. When the dose was lower...