The Study Of The Association Of ST3GalV (GM3 Synthase) And Tumor Metastasis

Metastasis leads to poor prognosis and it is the cause of death for patients with many types of cancer. A growing body of evidence supports crucial roles for glycans at various pathophysiological steps of tumour progression. Glycans regulate tumour proliferation, invasion, haematogenous metastasis and angiogenesis. Sugar chains are synthesized by glycosyltransferase. So it is very significative to research the association of glycolsyltransferase with tumor metastasis.We utilize a mouse mammary tumor model that consists of three distinct tumor cell lines (67NR, 4TO7, 4T1) which are derived from a single primary tumor that arose spontaneously in a wild-rival type BALB/cfC3H mouse. We research the expression of known glycosyltransferase by applying gene chip and real-time PCR in the three mammary tumor cells and find 19 glycosyltransferase with different expression, which probably are associated with development of tumor cells. The expression of ST3GalV distinctly increases in the highly metastatic 4T1. To determine whether ST3GalV plays a causal role in tumor metastasis, three ST3GalV gene knockdown 4T1 cell lines are established.The proliferation, migration and invasion assays detect the association of ST3GalV with pathophysiological processes that are important for cancer progression. The cells transfected with RNAi fragment proliferates with the same velocity. The migration and invasion potentials of ST3GalV gene knockdown cell lines decrease comparig with the control. The in vitro experiments indicate ST3GalV probably involves in cancer progression. In vivo assay directly detects the association of ST3GalV with tumor metastasis. The result indicates the weights of primary tumor have no difference but the metastatic nodules in lungs greatly decrease comparing with the control. The in vitro and in vivo experiments suggest that inhibition of ST3GalV expression by RNAi technology could inhibit the...