Preliminary Study On Plague Mucosa Adjuvant Vaccine

The majority infection factors enter the human body through the mucosa. Therefore, the delivery of a vaccine through the mucosa would have many potential advantages. Some researches indicated cholera toxin B subunit (CTB), proteosome and protollin were effective mucosal adjuvants. The study of PorA, PorB and Class 4 protein of proteosome adjuvant and functional mechanism of proteosome have vital significance for the developement of new mucosal adjuvants.In the recent, pneumonic plague which is transmitted person-to-person by the respiratory route or as the result of the spread of bubonic or septicemic plague by an untreated person, is the most fatal form of plague. Administration of adjuvant vaccines by mucosal routes is important and imperative way of inducing long term immune responses to protect pneumonic plague.In this report, outer membrane protein proteosome adjuvant was extracted from group B type 2 Neisseria meningitides; lipopolysaccharide (LPS), isolated from Shigella flexneri 2a, non-covalently complexed with proteosome formulated a novel protollin adjuvant; recombinant cholera toxin B unit (rCTB), PorA, PorB and Class 4 protein were derived from gene recombinance. Plague F1-V antigen was formulated with the above adjuvants, and immunized Balb/c mice intranasally. The studies reported here were conducted to determine which plague adjuvant vaccine was efficient and which adjuvant could be used as a mucosal adjuvant .1. Through different conditions, high purity proteosome was extracted from group B type 2 Neisseria meningitides. Polyacrylamide gel electrophoresis revealed three major bands corresponding to the PorA, PorB and Class 4 protein, with relative molecular weights 41 ku, 38 ku and 34 ku, respectively. The endotoxin, nucleic acid and capsule polysaccharide contents of proteosome were lower than 1% of it. Plague F1-V antigen formulated with proteosome adjuvant in a ratio of 4∶1, 2∶1, 1∶1, 1∶2 , 1∶4, and the plague adjuvant vaccines were used to immunize mice intranasally. The results demonstrated the adjuvant vaccines induced potent mucosal and systemic immune responses. 4∶1 ratio vaccine was most efficient compared with other ratio vaccines. Mice immunized intranasally with...