| Background and objectiveDengue virus (DEN) , a member of Flavivirus, was the pathogen of human dengue fever (DF) , dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS ) . Dengue virus comprises 4 serotypes. The transmission intermediary agents are Ae.aegypti and Ae.albopictus. The outbreaks of DF have been taken place in one hundreds of countries and areas of torrid and subtropical. Since 1978, several large-scale outbreaks of DF have been taken place in coastal areas of Northeast China such as Guangxi, Guangdong, Fujian, Taiwan and Hainan provinces. Statistics from the World Health Organization (WHO) showed that there were 50 to 100 million cases of DF, 250 to 500 thousand cases of DHF, among them 24 thousand died of infection. DF has been a severe public health problem all over the world, the study on dengue's molecular pathogenesis and the development of effective vaccines bore impending importance.Dengue virus, a member of Flavivirus, was unitary single strand RNA virus, hadn't DNA phase in its life cycle. It was quite difficult to study its characteristics directly on the genomic level, since the most powerful techniques of molecular biology, for example, mutation recombination and clone were used for DNA manipulation. Infectious transcripts technology brought a new way to solve the tough problem. It was a technique to reverse transcription RNA from cDNA. The RNA was build downstream on the RNA polymerase promoter. In the effective of RNA polymerase the full-length strand DNA was transcribed and then the transcripts were transfected into sensitive cell to obtain progeny virion. This technique provided a... |
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