Establishment Of A Cisplatin-Induced Human Nasopharyngeal Carcinoma Drug-Resistant Cell Line And The Relationship Between Multidrug-resistance And Apoptosis

Nasopharyngeal carcinoma (NPC) is endemic in southern China and Southeast Asian. Radiotherapy was the mainstay of conventional treatment. Recently, lots of clinical trials demonstrated that concurrent chemoradiation had achieved promising cure rates than radiotherapy alone, and that chemotherapy alone had showed a significant improvement in advanced nasopharyngeal carcinoma. However, the outcome of nasopharyngeal carcinoma is far from satisfactory. Due to the multidrug resistance, many patients in advanced stages eventually died because of the failure of chemotherapy. Cisplatin (CDDP) is one of the widely used therapeutic agents and acts both as a cytotoxic agent and as a radiation sensitizer. The acquired resistance to cisplatin often leads to the failure of treatment. Establishment of drug resistant cell line in vitro remains the main way for investigation in the mechanisms of chemoresistance. Presently, there is little information about the drug-resistant nasopharyngeal carcinoma cell line. In this study, we induced a cisplatin-resistant human nasopharyngeal carcinoma cell line HNE1/CDDP by continuously exposing and gradually increasing dose of cisplatin. The relationship between drug-resistance and apoptosis was also investigated so as to explore the possible mechanism involved in cisplatin-resistant nasopharyngeal carcinoma.