| Objective: To observe whether MSCs transplanted intravenously can migrate into impaired heart; to explore the effect of mesenchymal stem cell transplantation intravenously on cardiac function in heart failure rats and its mechanism.Methods: To establish a rat heart failure model, adriamycin was given intraperitoneally to 12-week old F344 rats (n=36) weekly at a dose of 2 mg·kg-1 body weight for 6 weeks. The survival rats (n=32) were then randomly divided into two groups, cell tranplantation group (n=16) and heart failure group (n=16). Another 10 normal rats were set as normal group, which received saline injection instead of adriamycin. MSCs were isolated from 8-week old F344 rats and cultured in vitro. Cells at the third passage were identified with a flow cytometer and were labeled with (4', 6-diamidino-2-phenylindole,dihydrochloride, DAPI) before transplantation. One week after the last administration of adriamycin, DAPI-labeled MSCs (3×106 cells) or culture medium was injected into rats (the cell transplantation group and the heart failure group, respectively) via femoral vein. Four weeks after the transplantation, cardiac function was evaluated with hemodynamic measurement. Hearts were harvested and observed with fluorescence microscope to identify whether MSCs can migrate into heart and differentiate into cardiomyocyte by immunofluorescence. Vessel numbers were calculated by VWF immunohistochemistry and collagen volum fraction(CVP) of left heart ventricle was calculated by Picrosirius red staining.Rusults: Cultured MSCs at the third passage were positive for CD44 and CD90 (99. 8%±0. 9% and 97. 7%±0. 4%, respectively), but negative for CDllb/c and CD34 (84. 2%±0. 2% and 97. 91%±0. 7%, respectively). Four weeks... |
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