Expression Of P33～(ING1b) In Primary Hepatocellular Carcinoma And The Relationship With P53,p21～(WAF1),Bax And Clinical Significance

Objective To study the expression of tumor suppressor gene p33^ING1b in hepatocellular carcinoma(HCC) and the relationship with p53,p21^WAF1and Bax,and to explore the role of p33^ING1b in the tumorigenesis of HCC and its clinical significance.Methods Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect p33^ING1b,p21^WAF1and Bax mRNA level in 55 HCC and matched adjacent tissues .Meantime,the expression of p33^ING1band p53 protein was measured with immunohistochemistry assay from the above mentioned 55 HCC and 20 normal liver samples.Results①The expression level of p33^ING1b mRNA in HCC was 0.410±0.175, and that in matched adjacent tissues was 0.529±0.203.There was very significant difference between them(P <0.001).Furthermore,p33^ING1b mRNA level was associated with pathological grades,envelope infiltration and lymph node metastasis(P <0.05),but not associated with sex,age,tumor size,tumor thrombus or liver cirrhosis(P >0.05);②The positive rate of p33^ING1b protein in HCC ( 38.2%)was significantly lower than that in normal liver tissues (70%)(P =0.015).Furthermore,p33^ING1b protein was associated with envelope infiltration and lymph node metastasis(P <0.05),but not associated with sex,age,tumor size,pathological grades,tumor thrombus or liver cirrhosis(P >0.05);③The positive rate of p53 protein in HCC ( 67.3%)...