Effect Of H202 On Cultured Melanocytes And The Relationship Between Oxidative Stress And Vitiligo

Vitiligo is a depigmenting disease of the skin ,characterized by destruction of MC in lesions of vitiligo , resulting in patches of depigmentation. In China, the incidence of vitiligo in patients is about 1% to 2%. It can condition the behavior of people suffering from vitiligo and can deeply affect their personal relationships. The etiology is still unknown and pathogenesis has not been completely clarified so far, various hypotheses have been proposed, they include the genetic hypotheses, the autoimmune hypotheses , the autocytotoxic hypotheses, the neural hypotheses, and the lack of microelement hypotheses.In recent years , Increase levels of H₂O₂ were demonstrated in the epidermis of both lesional and nonlesional skin of vitiligo patients, H₂O₂ levels increase in response to oxidative stress. An imbalance of antioxidants was found in the epidermis, correlated to an increased reactive oxygen species(ROS) production. ROS can interact with macrom olecules such as proteins, membrance lipids and nucleic acids, and fina lly cause cells damaged.In this study , Normal melanocytes were cultured with H₂O₂ in different dosages(0-0. 5mmol/L) and different time(0—60minute), the appearance and function of the cells were analysed .The aim of this study was to evaluate the effect of H₂O₂ on the melanocyte in vitro cultured and the relationship between oxidative stress and vitiligo. Part One Culture and biological characteristics of melanocytes Objective To indentify the biological characteristics of MC cultured in...