The Separation, Purification And Pharmacological Action Research Of Gynostemma Pentaphyllum Polysaccharides

Gynostemma pentaphyllutn (Thunb.) Makino, a member of Cucurbitaceae family, is a kind of herb. There are 16 species and 3 varieties of Gynostemma pentaphyllum in the world, and 15 species and 3 varieties in China. They are distributed over large areas in the south provinces of China, especially in Yunnan province. In the 90s of last century, there were many reports about Gynostemma pentaphyllum, but they focused mainly on Gynostemma pentaphyllum saponins and only a few about Gynostemma pentaphyllum polysaccharides. Raw polysaccharidebs but not homogeneous polysaccharides was used in the previously research of Gynostemma pentaphyllum polysaccharides .According to the reports, the main pharmacological action of raw Gynostemma pentaphyllum polysaccharides included the action of anticancer and the action of enhancing immunity. In this papers, we separated and purified the raw Gynostemma pentaphyllum polysaccharides and obtained two homogeneous polysaccharides. We studied the structure and pharmacological action preliminarily. The main contents of the papers are as follows:1. The optimization of extraction method of raw Gynostemma pentaphyllum polysaccharides. First, monosaccharides, oligosaccharides and other micromolecule matters were removed by ethanol, then orthogonal design method was used to optimize the extracting conditions. Considering the extraction efficiency and quality of polysaccharides, the final optimization extracting method was A₂B₁C₃D₂.2. The separation and purification of raw Gynostemma pentaphyllum polysaccharides. First pectin was removed by calcium chloride from raw...