Expression Of MMP-13,TIMP-2 In Rabbit Liver With Nonalcoholic Steatohepatitis And The Effect Of Intervention With Pioglitazone

Objective: 1.Prepare rabbit model of non-alcoholic fatty liver disease (NAFLD) by cholesterol-rich diet . Pioglitazone was given from the ninth week in group C. At the end of thirteenth week, all the rabbit were sacrificed. TC, TG, insulin resistance index, histological changes, MMP-13, TIMP-2 expression of liver were measured . 2. In order to investigate the implications of matrix metallopro teinase-13(MMP-13)and tissue inhibitors of metalloproteinase-2 (TIMP-2)in the development of liver fibrosis in experimental nonalcoholic steato-hepatitis (NASH), dependablity between the hepatic pathological change and the variance were evaluated. 3. To investigate Pioglitazone's protection effectiveness that in the development from NAFLD to hepatic fibrosis and the mechanisms.Methods: 15 rabbits were randomly allocated into the normal group (group A), the model group(group B)and the Pioglitazone group(group C).In group A rabbits fed with normal diet were taken as controls; NASH model was established by feeding rabbits with cholesterol-rich diet in group B, and in group C Pioglitazone (10 mg. kg^-1d^-1) were given by intragastric administration from the beginning of the ninth week . All the rabbits were sacrificed at the 13th...