| Objective: To study the effects of chronic alcoholism and withdrawal on learning and memory and NMDA receptor subunits NMDAR2B expression in hippocampus of rats, Methods: Fifty-eight wistar male rats were randomly divided into control group and experimental group, and the experimental group were divided into given 15% alcohol group ,given 50% alchol not withdrawal group , withdrawal 36h group ,withdrawal 3d group ,withdrawal 1w group and withdrawal 2w group. Experimental group were poured into 50% or 15% alcohol 10ml/kg/d,and control group were poured into physiological saline of same quantity for 8 weeks. After completion of pouring the alcohol to experimental rats , all groups of rats were tested by water maze .Afterwards, the brain tissue sections were performed by immunohistochemistry to demonstrate the expression of NMDAR2B immunoreactivity in the hippocampal CA1,CA3 and dentate gyrus then they were analyzed by computer-assisted image analysis system.Results: (1)Withdrawal can be observed in the given 50% alcohol group at the eighth week;(2)by comparing with the rats of control group and the given 15% alcohol group, the latent periods to search platform was prolonged in the given 50% alcohol group and the 2w withdrawal group;(3)by comparing with the control group the expression of NMDAR2B was significantly up-regulated in the given 50% alcohol group and the given 15% alcohol group;(4)by comparing with the rats of control group ,the expression of NMDAR2B was significantly up-regulated in the 36h,3d and lw withdrawal group and got to maxium at the 3th day then decresed graduately from lw; (5)by comparing with the rats of control group ,the expression of NMDA2B was significantly down-regulated in the 2w withdrawal group.Conclusion: Chronic alcoholism might decrease the ability of learning and memory; one of these mechanisms of chronic alcoholism and withdrawl might be related to up-regulation for expression change of NMDA receptor subunit NR2B;the ability of... |
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