Serological Diagnosis Of Schistosomiasis Using Recombinant Signaling Protein 14-3-3 And 26kDa Glutathione-S-transferase Of Schistosoma Japonicum

Objective: To approach the poteintial of the recombinant signaling protein 14-3-3 (rSjl4-3-3)and 26kD glutathione-S-transferase(GST) of Schistosoma japonicum in the diagnosis of schistosomiasis. Methods: The SjGST gene was amplified from recombinant plasmid pBK-CMV-SjGST by Polymerase chain reaction(PCR) and subsequently cloned into expression vector pET28a(+). The rSj14-3-3 and rSjGST were expressed in E. coli and purified through the collumn of affinity chromatography with Ni²⁺(nickel sulfate, NiSO₄). The rSj14-3-3 and rSjGST antigens were subjected to the serological diagnosis in the patients with acute and chronic schistosomiasis japonica using indirect ELISA. The sensitivity and specificity of the recombinant antigens used in ELISA (rSj-ELISA) were compared in parallel with the traditional methods of SEA-IHA and COPT. In addition to the sera of patients with acute and chronic schistosomiasis, the detected samples include the sera of individuals with clonorchiasis and hookworm infections and those from the areas with and without schistosomiasis. A paralled tests were also performed using the adult worm antigens( SjAWAs) in ELISA. Results: The size of PCR products of SjGST was approximately 670 bp, which is...