The Establishment Of A Canine Model Of Portal Hypertension With Esophageal Varices And Study On Durg Action Of Somatostatin, Octreotide

Background: Hemorrhage from esophageal varices remains the most dreaded complication of liver cirrhosis with portal hypertension. To establish a canine model of portal hypertension with esophageal varices, is benefit to study the hemorrhage mechanisms of esophageal varices and evaluate the effect of concerned drugs. Objective: To establish a canine model of portal hypertension with esophageal varices, and explore the effects of somatostatin and octreotide on portal pressure and vasoactive substance.Methods: â‘  Preparation for animal model: Twelve adult mongrel dogs were used to develop the esophageal varices model. A laparotomy was perfomed using sterile technique, constructed a side-to-side portacaval shunt during the IVC and portal vein(PCS); an ameroid constrictor was placed around the cephalad part of the portal vein(AC), the portal vein cross sectional area was initially reduced by 50%; the cephalad part of the IVC was double ligated and cross-sutured(IVC ligation). Panendoscopies were done regularly to observe the development of esophageal varices and abdominal veins. A second laparotomy was performed six weeks later, the portal vein was ligated cephalad to the ameroid constrictor. Potal pressure was measured four times during the model establishment, before the portacaval shunt, after the IVC ligation, before the portal ligation in second operation and after the portal ligation.â‘¡ Pharmacological experiment: A second sterile laparotomy was performed six weeks after the first operation. Eight surviving dogs were divided randomly into somatostatin group and octreotide group. While an intravenous infusion of somatostatin or octreotide,...