A Study On The Tumor Marker For Diagnosing Ovarian Cancers By Tissue Microarray And Preparation Of Monoclonal Antibody Against Antigen Associated With Ovarian Cancer

Objective To assess the value of CA125 and Ep-cam for diagnosing and differenttiating ovarian neoplasms by constructing and applying high-throughput tissue microarrays. To establish the monoclonal antibodys against antigen assosiated with ovarian cancer, study their charactorization. Methods The ovarian tissue chip was prepared, CA125 and Ep-cam were detected with immunohistochemical staining methods. The results were analyzed by referring to the pathology of the specimens to calculate the sensitivity, specificity, accuracy and expected positivity of the single monoclonial antibodies (MoAb) tests and the combinations of the tests; Using hybridoma techique, we prepare SK-OV-3. The hybridoma cells are obtained by fusing spleen cells of Balb/c mice to myeloma cells SP2/0 and culured in the HAT selective medium. The positive clones are screened by indirect ELISA and the limited dilution methods are used to select anti- Antigen Assosiated With Ovarian Cancer McAbs. McAb class and subclass are identified by Immunotype? kit. The Western blotting method is analysed the specificity of McAb. Results The specificity of CA125 test was 86.67%, the value would be increased to 93.33% when the positive result of Ep-cam was considered together, with the rate of false positivity being 3%; One of hybridoma cells secreting Antigen Assosiated With Ovarian Cancer mAbs is obtained, its subclass is IgM.The antigen recognized by 3c6 was a protein with a molecular weight of 67 KD. Conclusion Joint detection of CA125 and Ep-cam will increase the specificity of the immunoassay and lower its rate of false positivity. The combination of CA125 and Ep-cam is of high practical value for diagnosing ovarian cancer; One hybridoma cell line, which secretes monoclonal antibody against Ovarian Cancer, was established.