The Role Of JWA Involved In Oxidative Stress In HeLa Cells Induced By Hydrogen Peroxide And Benzo(a)pyrene Exposure

Oxidative stress, an intracellular imbalance toward the pro-oxidant side of the pro-oxidant/antioxidant homeostasis, occurs in several human diseases. It may very important to understand the oxidative stress associated molecular events in cancer cells. As a very popular representive pro-oxidant of reactive oxygen species (ROS), hydrogen peroxide (H₂O₂) was often used as a stressor in the study of oxidative stress. It is indidicated that H₂O₂ can easily penetrate into nucleus of the target cells and affects the stabilization of DNA, which then leads to the strand breakage and DNA damage. Benzo(a)pyrene (B(a)P), a prototypical member of this class of chemicals, has been extensively studied for its toxic effects in laboratory animals and human populations. Reaserchees have shown that the toxicity of B(a)P is usually mediated by its oxidative metabolism to reactive intermediates that interact with macromolecules leading to alterations in target cell structure and function. Benzo(a)pyrene 7,8-diol-9,10-epoxide (BPDE), a putative carcinogenic...