| After comparing different lixiviation methods of green tea ,using time after time and multiplestep lixiviation method to lixiviate tea samples,then determined GAB A, caffein and other general ingredients in tea soup.Constrasting test results and according to the principle of high GABA, lower caffeine and other ingredients relatively high,chose F915h 水潦和 F615h 水潦 (Fuxuan 9 and Fuyun 6 .dealt with 15h absence of oxygen and left off caffein ) as acute toxicity evaluation samples , F615h 水潦, F615hs (Fuyun 6 dealt with 15h absence of oxygen) and F6XoSn(general green tea) as assisting blood pressure reduction function evaluation samples.Consult assisting blood pressure reduction fuction and acute toxicity evaluation standard in our country ,the study used spontaneously hypertensive rat and white mice (KM) as test animals to evaluation acute toxicity and assisting blood pressure reduction function of these tea samples ,also primarily discuss the blood pressure reduction mechanism.The study can ensure these teas put into market safely, upgrade tea value, drive to tap of natural and little side-effect health food. The results is as followings:1. Pouring larger dose tea soup one-time into white mice from mouth to stomach every day, every group mice drinking water , ingestion, nerve and activity were all normal after two weeks and no mice was died.It could not draw definite LD50(mg/(kg.bw)) ,but according to the largest pouring dose ,we could judge that LD50>5000mg/ (kg.bw) of the two tea samples,according to chemical substance acute toxicity classification standard,we can say that these tea samples are all innocuity.2. Pouring 0.42g/ (kg.bw) F615h 水潦, F615hs,1.05g/(kg.bw)F615h 水潦 and 0.8mg/ (kg.bw) GABA standard solution into SHR stomach every day ,40ds later, systolic pressure of SHR reduced obviously compared to initial value and 1.05g/(kg.bw) F615h 水潦 had great effect. 0.42g/(kg.bw) F6XoSn and 0.21g/(kg.bw) F615h 水潦 also could reduce blood pressure ,but it did not have... |
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