Detecting Of Gene Rearrangement In Peripheral Blood Of Non-Hodgkin's Lymphoma Patients And Its Clinical Significance

Background and ObjectivesIn recent years, the incidence of Malignant lymphoma (ML) is increasing. The incidence of Non-Hodgkin's lymphoma (NHL) is 89.1% in all ML and it is about 7 times more than that of Hodgkin's disease(HD) in China. In clinic, there is some difficulty with routine histomorphology and immunohistochemistry methods in diagnosing, staging, evaluating prognosis and determining whether and how long the patients should be treated. Therefore, it is important to set up a comprehensive molecular level assessment system of diagnosis, treatment and prognostic for NHL.NHL can obtain complete response(CR) by chemotherapy,but there will be below 10~6 tumor cells in patients body . The major cause of relapse is that there are some residual tumor cells in patients body. They are called minimal residual disease (MRD) . It can not be found by the routine detecting assays. So a more sensitive method shoud be designed to detect it.The rearrangement styles of antigen receptor genes of every B or T lymphocyte are completely different. During the dedifferentiation and development of lymphocytes, if a lymphocyte at some stage transforms into a malignant cell and then proliferates monoclonally, malignant disease will be generated subsequently and the gene encoding of antigen receptor is coherent.There are many ripe lymphocyte in...