Study Of Specific Cytotoxic T-lymphocyte Effect Induced By Dendritic Cells Transfected With Total RNA Of Ewing' Sarcoma

Ewing' sarcoma is one of primary malignant bone connective tissue tumors of children. The serious malignant degree and fast growth cause high death rate. In spite of the good effect of radiotherapy and chemotherapy on patients of Ewing' sarcoma survival rate in 5 years, there are half number of patients of Ewing' sarcoma who succumb to metastasis. It provided new ways for immunotherapy, especially initiative of tumors that human tumor antigen and their genes was found and confirmed. It's the hot spot of present research that tumor specific antigens (TSA) or the vaccine of their related antigen was acted directly. 95% of Ewing' sarcoma include characteristic chromosome translocation t(11;22)(q24;q12), the reason to form EWS-FLI1 fusion gene, which is the primary factor of Ewing' sarcoma family's genesis and development, and the marker of Ewing' sarcoma family's diagnosis, treatment and prognosis. So, the generation of specific vaccine to induce the immune reaction which aims directly at EWS-FLIl could be a kind of new idea to cure Ewing' sarcoma.Dendritic cells (DC) are professional antigen presenting cells. It...