Studies On Repair Of Rabbit Radial Bone Defect By Tissue Engineering Bone Which Has Been Constructed With Mesenchymal Stem Cells

[Objective] To create a kind of methods by which rabbit's marrow stromal cells can be cultivated in vitro and its growth rule and morphology also can be observed. To explore its biocompatibility with PLGA and BMG .Then the tissue-engineered bone was constructed when BMSC was seeded onto PLGA or BMG and its feasibility and ability to enhance segmental bone defect healing of rabbit radius were explored.[Methods] (1) Bone marrow was obtained from long bone of young rabbit and mononuclear cell layer was isolated by density gradient centrifugation. Respectively undertake primary culture and serial subcultivation and observe the growth characteristics and growth curve of every generation cell, at the same time analyze various kinds of staining of slide to evaluate the feasibility of BMSC to be used as...