Experimental Study On Cultivation And In Vitro Inducement Differentiation Of Spinal Cord-derived Neural Stem Cells

Spinal cord injury (spinal cord injury, SCI) is commonly encountered in clinical.. Traumatic spinal cord injury can result in severe damage, leading to tetraplegia, or worse. The finding of NSCs (neural stem cells, NSCs) will bring splendid prospective and enhance our confidence in overcoming spinal cord injured-disease. This study is about in vitro cultivating, proliferation and differentiation of spinal cord-derived NSCs, it provide convenience and aliable seed cells. this reaearch lay an experimental foundation to the next research of transplant therapy to SCI.Part 1 To investigate the character and regularity of cultivation and in vitro inducement differentiation of spinal cord-derived NSCs from the embryonic rats. we isolated clone cells from E12 fetus rat's spinal cord through the methods of microscopic dissection,serum-free cell culture,these cells were subcultured in vitro. We also investigated the effects of Leukemia inhibitory factor(LIF) on the proliferation and differentiation of cultured spinal cord-derived NSCs.the neural cell phenotypic antigens were identified by means of immune fluorescent assays,and the ratios between phenocyteswere calculated. The result we found it that:1. The spinal cord-derived NSCs are capable of proliferation, repeated subculture.In the presence of epidermal growth factor(EGF) and basic...