Underlying Molecular Mechanisms Of Apoptosis And Cell Cycle Arrest Induced By Resveratrol In Medulloblastoma Cells

Objective: 1) To study the status of caspase 8 in human medulloblastoma cells in vivo. 2) To explore the expression patterns of caspase 8, cyclin A, cyclin B1 and cdc25A in human medulloblastoma cell line UW228-2, -3. 3) To analyze the effects of resveratrol on the expressions of caspase 8, cyclin A, cyclin B1 and cdc25A and the role(s) of c-Myc antisense oligonucleotides (ODN) in regulating the expressions of these cyclin A and cyclin B1 genes. 4) To elucidate the potential action mechanisms of resveratrol on medulloblastoma cell lines. 5) To provide a new laboratory evidence and theoretic basis for better treatment of human medulloblastoma.Methods: Human medulloblastoma cell line UW228-2, -3 was cultured in DMEM (Dulbecco's Modified Eagle's Medium) culture medium containing 10% Fetal Bovine Serum. The cells were treated with resveratrol (100μM) when cells were in good state. Then observe the effects of resveratrol-induced differentiation and apoptosis of medulloblastoma cells. Put sterilized cover slips in the dishes before cell seeding for cell shape observation or immunocytochemical analysis was performed. After above treatments, cover slips were collected and fixed with 100% cold acetone. The cell proliferation situations of experimental groups were examined through Trypan Blue counting method. Immunocytochemistry (ICC), western blot and RT-PCR were used to analyze the expression levels of caspase 8, cyclin A, cyclin B1 and cdc25A of individual experimental groups. Paraffin embedded tissue blocks of ten medulloblastoma cases and their surrounding noncancerous counterparts were collected from the Pathological Department, the First Affiliated Hospital of Dalian Medical University. The tissue microarrays (TMA) were...