Application Of Microchip In The Research On The Relationship Between GRP78 And Drug Resistance In Lung Cancer

Lung cancer (LC) is still among the most frequently occurring malignancies and is the leading cause of death in the world. Chemotherapy is the main treatment of lung cancer, but it often failed because of drug resistance. The complexity of drug resistance in human cancer strongly suggests the involvement of multiple pathways. One mechanism, both intrinsic and acquired, is the result of genetic alterations within cancer cells. Another mechanism may result from environmental conditions that occur naturally in solid tumors. Thus, there is an urgent need to explore other treatment options in human lung cancer.Because of poor vascularization, solid tumors usually contain regions undergoing glucose starvation and hypoxia, and expression of a series of glucose-regulated stress proteins (GRPs) was commonly enhanced in such stress. The best characterized GRP is GRP78, which facilitate proper protein folding, maintain protein in a folded state, and prevent protein folding, intermediates from aggregating, suggesting that GRP78 may play a critical role in maintaining cell viability. There have been many reports showing the protective role of GRP78/BiP as a molecular chaperone against ER stress induced cell death in breast cancers, colon cancers and ovarian cancers. The relationship between expression of GRP78 and drug resistance in NCI-H460 lung cancer cell line has not been well defined.The direction of modern chemical analysis instruments in 21th century is to push for integration, minimization and portability. Microfluidic devices, offer a number of advantages such as reduced sample and reagent...