Exploration Of Oxidative Damage Induced By High-lipid Diet On Spontaneously Hypertensive Rat And The Possible Mechanisms Underlying Its Blood Pressure Change

Essential hypertension (EH) is the pathology characterized by constant blood pressure increasing which is caused by multiple genetic and environmental factors. The morbidity of this disease is closely associated with age. About 25% to 35% of adults suffer the EH, among them most are old people with age above 70. EH could be complicated by other cardiovascular diseases, in which hyperlipidemia is a close twin. Researches show that unbalance of lipid metabolism frequently co-exists with hypertension. The total cholesterol of EH patients is much higher than that of normal ones[Williams RR,1988; Julius S , 1990]. Epidemiology investigation about the relationship between abnormal blood lipid and hypertension also confirmed that the blood cholesterol level paralleled with blood pressure[Castelli WP, 1986; Laurenzi M, 1990]. Against this background, to study hypertension complicated with hyperlipidemia is more practical and target-oriented, because it reflects the pathologic characteristics of EH patients. There are, however, few direct evidences of the relationship between hypertension and its complications. In this paper, the author established hypertension rats complicated with hyperlipidemia to investigate the oxidative stress impact of high-lipid diet on target organs of spontaneously hypertensive (SHR) rats and explore the he possible mechanisms underlying the blood pressure change in hyperlipidemia condition of SHR.There are some shinning points in this paper. First, research about the fibrinolytic system in rats is quite few at home and abroad. Especially, there is no report in spontaneously hypertensive rats (SHR). In addition, this research investigated the NO/ET system and fibrinolytic system in the hypertension complicated with hyperlipidemia for the first time. Second, on the methodology of smooth muscle cell culture, this research doesn't employ the traditional ways. The author adopted the artery ring culture method after trials and errors. In addition, the cultured cells are treated with the serum extracted from model rats. Thus, the in-vivo pathology of rats can be better emulated and the effect is proved to be quite good.This dissertation is divided into three parts:Animal level: Establish hypertension combined hyperlipidemia rats models and explore the oxidative damage caused by high lipid diet. WKY and SHR rats are used in the experiment and grouping is as follows: normal group (N), simple high lipid group (N+L), simple hypertension group (S) and complication group (S+L). 8 weeks...