| Background: Pathological scars may be divided into two kinds: hypertrophic scars and keloids. They are all made up of collagen fibers, cells and new-born blood vessels with the characteristic of fibroblasts' hyperplasia and collagen' s over-deposit. Whereas the collagen is secreted mainly by fibroblasts, which, it is concerned , has a close relationship with the formation of pathological scars. Along with the rapid development of cellular and molecular biology in recent years, the comparison of gene profiling of normal skin with hypertrophic scars has identified some gene segment responsible for the formation of scar. Such are important evidences for our study. SELDI is a new technology which developed after gene chip. SELDI combines protein chip technology with Mass Spectrometry . It has some merits such as high quantum, high efficiency, good repeatability of chip , and high sensitivity of Mass also. The development of knowledge and technology in proteomics has provided a powerful method in studying diseases.Objective: To screen and evaluate protein biomarkers for the fibroblastic cells of hypertrophic scar and normal skin. By Ciphergen's SELDI-TOF-MS , all different protein spots were detected from the protein chips. From the point set, some special spots were picked out as the part of desired protein fingerprint.. Combining with bioinformatics, a specific patterns was established successfully to discriminate hypertrophic scar from normal skin.Methods: The fibroblastie cells from eighteen cases of hypertrophic scar and six cases of normal skin were cultured individually in vitro. |
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