| Objectives:Stem cell therapy of myocardial infarction is an ideal one, which can increase the population of functional cardiomyocytes and improve the heart function. It has being at initial stage of basical and clinical research, and it is also the focus of heart researchers all over the world. P19 carcinoma cell is an embryonic original stem cell, which has being studied well and highly valued by researchers. Oxytocin, a female hormone, has been discovered to have many other functions recently, including the function of promoting cardiogenesis. Our research is try to induce p19 carcinoma cells to differentiate into cardiomyocytes by oxytocin (OT) and observe the induced cells with methods of immunocytochemical staining,laser scanning confocal microscope(LSCM) and transmission electron microscope(TEM), and provid the data of stem cell therapy for treating myocardial damage in basical research.Methods:1 Inducement and differentiation of p19 cellsThe p19 cells of log phase were digested into cell suspension by 0.25% Trypsin and 0.2% EDTA. 2ml cell suspension (2×10~5cells/ml) was suspension cultured for 4 days... |
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