Research About The Expression Of P73 And P15 As Well As DNA Ploidy In Giant Cell Of Tumor Of Bone

Objective:This paper was designed to study the correlation of the expression of p73, p15 protein as well as DNA ploidy and the biology behavior of Giant cell tumor of bone (GCT), and the interaction among the p73 , p15 protein and DNA ploidy, and the effect that p73 and p15 bring to the cell cycle, thus on the one hand to determine the value of p73, p15 protein as well as DNA ploidy in prognosis for GCT, on the other hand to realized the diversification and action of p73 and p15 in GCT.Methods: We select 30 GCT paraffin samples which had been excised in surgery between 2000 and 2005 from the pathology department of the second hospital of Shanxi medical college. The specimen group divides into 16 cases of grade I, 8 cases of grade II , 6 cases of grade III as the Jaffe grading, and 6 cases of stage1 , 13 cases of stage 2, 11 cases of stage 3 as Ennecking's stage; There were 8 cases recurred and 22 cases not recurred, 4 cases metastasized and 26 cases not metastasized. We detected the p73, p15 protein by using S-P immunohistochemistry and DNA ploidy by using flow cytometric DNA analysis.Results: p73 , p15 protein show itself the fine-grain shape are mainly located in the nucleus, also may be seen in the cytoblastema. Both of them expressed mainly in the mononuclear stroma cell. The positive expression rate of p73 protein and the p15 protein in the GCT samples are respectively 40% (12/30) and 56.7% (17/30); The expression of p73 and p15 protein correlate with Ennecking's stage, the expression intensity increases along with the advances of Ennecking's stage (P < 0.05), and is higher in the recured group than not recured group(P < 0.05, P < 0.01). In addition, the expression of p15 protein is higher in the metastasized group than not (P < 0.01). In specimen group, 14 samples(46.7%) is DNA euploid and 16 samples (53.3%) is DNA heteroploid. DNA ploidy correlate with the Ennecking's stage and whether recurred. The heteroploid rate in recured group is higher than not recured (P < 0.05), and increases along with the advances of Ennecking's stage (P < 0.01), but there is no discrepancy during the two groups that metastasized and not (P > 0.05); The expression of p73 , p15 protein as well as DNA ploidy does not correlate with the Jaffe's grading (P > 0.05); The expression of P73, p15 protein is both higher in the heteroploid group than the euploid group (P < 0.05); The G0/G1 stage cells is lower in p73, p15 protein positive group than in the negative group (P < 0.05, P < 0.01), and the S stage cells is higher in the positive group(P < 0.05, P < 0.01); The expression of the p73 protein and p15 protein present the correlation after the Sperman relevance examination (P < 0.05).Conclusions: There is a statistically significant correlation between the expression of p73, p15...