Protective Effection Of Rosiglitazone Sodium On The Survival Pancreatic Beta Cells Of Diabetic Rats

Objective To explore the regulated effection of rosiglitazone sodium on survival pancreatic beta cell of diabetic rats. Methods 48 rats were randomly assigned as control group(group A) and diabetic control group(group B) and rosiglitazone sodium-treated diabetic group(group C). STZ were administered intraperitoneally at a dose of 65mg/kg body weight for both group B and group C and Seventy two hours after that, fasting blood glucose(FBG) levels were assessed. Of the group B and group C,those with FBG≥16.7mmol/L were served as diabetic models. Rosiglitazone sodium(5mg/kg.d-1)were applied each day among those animals of group C by intragastric administration. One-,two-,four-, seven-, and ten weeks after the administration of rosiglitazone sodium ,both group B and C were executed 4 animals respectively. Pancreas samples were removed and preserved for subsequent use. Fasting blood samples were collected for the assessment of FBG, insulin(INS) levels. Histopathology and ultrastructrure of pancreatic islet were observed by light microscopy and transmission electron microscopy. The expression levels of INS, Ki67 in pancreatic islet were measured by immunohistochemistry. Result In group C, the levels of blood INS were increased over time, and the FBG levels were decreased gradually.The size of pancreatic islet were enlarged and the beta cell mass were raised in those animals of group C. The levels of expression for INS in the islet were significantly higher in group C than that of group B.A lower levels of expression towards Ki67 in pancreatic islet were observed only in group C but not any was noted in the other two groups. Conclusion...