The Expression Of HBD-2 And HBD-2mRNA In Salivary Gland Of Sjogren's Syndrome

Objective: The study was aimed to explore the expressions of HBD-2 and HBD-2mRNA in salivary glands of patients with Sjogren's syndrome(SS), and to explain the correlation between HBD-2 and clinical, clinicopathologic features.Methods: Clinical data and specimens were obtained from 30 cases suffered from Sjogren's syndrome and 10 cases from normal salivary glands in the Stomatology Hospital of Guangxi Medical University from January 2000 to February 2006. The streptaividin perodaxe immunohostochemical and in situ hybridization method were used to detect the expressions of HBD-2 and HBD-2mRNA respectively. Correlations between HBD-2 and Chisholm's score or organic manifestations were investigated. SPSS10.0 was used for statistical analysis.Results: HBD-2 was observed in salivary glands from patients with Sjogren's Syndrome, but few HBD-2 positive cells could be seen in salivary glands in the normal specimens. The positive expression was mainly in the cytoplasmicacinar of ductal epithelial cells in Sjogren's syndrome but few was in normal salivary glands'ductal epithelial cells(p=0.001). Positive cells of acinar cells and lympho -cyte were rarely observed both in SS and normal specimens; HBD-2 mRNA was also observed in salivary glands'ductal epithelial cells from patients with Sjogren's syndrome, but few was observed in ductal epithelial cells of normal salivary glands(p=0.024). Positive cells of acinar cells and lymphocyte were rarely observed both in SS and normal specimens. The high score of Chisholm's score and SS with other organic manifestations were also correlated with a higer...