Study On The Effect Of Wogonin On The Telomere Length, Telomerase Activity And Apoptosis Of Human Ovarian Cancer SKOV3 Cellular Xenograf In Nude Mice

objective: Regulating telomerase activation is an ideal way to treat cancer.Current studies show that wogonin has the attributes of anti-oxidation and suppress the growth of tumor cells.This study aimed at evaluating the effect of wogonin on telomerase activation of implanted human ovarian cancer cells SKOV3 in nude mouse. The expression of Bax,Bcl-2 and hTERT,the activation of telomerase, the length of the telomere were detected. The relationship of them was also explored.Method: Southern blotting hybridization and gel electrophoresis were used to assay the length of the telomere.The change of the telomerase activation was examined by Telomerase Regulation-associated Protein-Polymerase Chain Reaction(TRAP-PCR)-silver staining.The expression of telomerase gene hTERT was observed by RT-PCR. The expression of Bcl-2 and Bax were detected with immunohistochemical method, terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) technique were applied to detect the index of cell apoptosis.Nude mice with implanted human ovarian cancer SKOV3 cells were randomly divided into five groups,viz the high dose group(group A),low dose group(group B),normal control group(group E),cisplatin therapy group(group C),and combined therapy group (cisplatin and wogonin,group D).The weight of nude mouse and the volume of tumor were regularly examined.Then DNA,RNA and Protein were extracted from the tumor tissue.Results:(1)wogonin inhibited the growth of SKOV3 cell of human ovarian cancer.The inhibiting concentration of 50% was 90μg/ml after 24 hours...