The Correlation Between T-786C Polymorphism Of Endothelial Nitric Oxide Synthase Gene And Coronary Artery Disease

Objective To investigate the correlation between T-786C polymorphism at promoter in the 5'-flanking region of the endothelial nitric oxide synthase gene and the extent of disease or degree of severity in coronary arteries and coronary artery disease in Chinese Hans, in order to provide evidence for coronary artery disease in etiopathogenisis.Methods The primer was designed based on the site of T-786C in the 5'-flanking region of endothelial nitric oxide synthase gene. 136 coronary artery disease and 77 controls were defined by coronary angiography (CAG). The number of T-786C polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism(PCR—RFLP) method .Results (1)The frequency of TT, TC and CC genotype were 72.8%, 25.7% and 1.5% respectively in patients with coronary artery disease group, but they were 92.2%, 6.7% and 0 respectively in control group. The frequencies of TC and CC genotype were significantly higher in coronary artery disease group than in control ( x~2= 11.5, P=0.001);(2)The frequency of C allele gene were significantly higher in coronary artery disease group than in control ( 14.3%vs3.9%, x~2= 11.3, P=0.001);(3)Multivariate logistic regression analysis showed that T-786C polymorphism in the 5'-flanking region of endothelial nitric oxide synthase gene was a independent risk factor for coronary artery disease (odds ratio 5.261, 95%CI:2.010—13.768);(4)The frequency of TC+CC genetypes were 21.4%, 25.0% and 33.3% ( *2=1.83, P=0.40) respectively, and the frequency of C allele gene were 10.7%,12.5%,18.5% ( *2=2.66, F=0.265) respectively in 1, 2, 3 stenosed coronary arteries. The differences among these three groups are not significant in statistics .Conclusions T-786-*C mutation at promoter in the 5'-flanking region of eNOS gene are related to angiography defined coronary artery disease in Chinese Hans. It's a inherit risk factor for coronary artery disease and it appears a independent factor to other classics risk factors;(2) There was no correlation between T-786C polymorphism and the extent of disease or degree of severity in coronary arteries.