The Analysis Of The DNA Fingerprinting Of Intestinal Flora In IBD Patients

Inflammatory bowel disease (IBD) is a group of chronic enteric inflammatory diseases without definite etiopathogenisis. Microorganism plays an important role in pathogenesis of IBD. But no specific pathogenic microorganism has been found related to IBD so far. The number of enterobacteria species could be cultured in vitro only accounts for small percent of the total number of enterobacteria species. DNA fingerprinting based on PCR technology could be used to analyze the makeup of intestinal flora. Enterobacterial Repetitive Intergenic Consensus (ERIC) sequence is a kind of reverse and repeated sequence mostly located in the enterobacteria genome that is 126bp in size and highly conservative. ERIC-PCR generates multiple distinct amplification products of sizes ranging from approximately 50 to 3000 bp. Each kind of bacteria has special electrophoretic bands with different number and the main band could be appeared stably and repeatedly. The unique location of ERIC elements in bacterial genomes allows discrimination at genus, species and strain levels based on the electrophoretic pattern amplification products. In this study, we set up the DNA fingerprinting of IBD patients and healthy persons based on ERIC-PCR technology. We have identified the DNA fingerprintings of IBD patients and the healthy persons, and a significant difference was noticed between them.There are a lot of bands in the DNA fingerprinting of the healthy group but fewer in the DNA fingerprinting of the IBD patients. Strikingly, a same band of DNA fingerprinting was noticed in IBD patients. So the variaty of intestinal flora of healthy patients is more than it of IBD patients.The unique band might be the sequence of specific bacterium. Or it might be the sequence of different bacterium and the mixed sequence.