Study Of Antioxidation Role Of Glutathione On Intestine Ischemia Reperfusion Injury In Rat

IntroductionTo investigate the antioxidation role and protective effect of Glutathi-one on intestine after intestinal ischemia reperfusion injury in rats in vivo.Experiment materials and methods36 adult Sprague— Dawley rats weighting 250g—270g were anesthetized with 10% chloride hydrate (3ml/kg) and subjected to intestinal ischemia (1 hour of superior artery occlusion along) followed by 60 minutes or 120 minutes of reperfusion. Animals were devided into 6 groups : A . sham operated controls 60 minutes (n = 6 laparotomy , 0. 3ml NS at the time correspond to 30 minutes before reperfusion) , B ischemia and reperfusion 60minutes (n = 6, 0. 3ml NS 30 minutes before reperfusion and reperfusion for 60 minutes), C. Glutathione pretreatment 60 minutes (n ==6,0. 3ml 20% Glutathione NS solution 30 minutes before reperfusion and reperfusion for 60 minutes), D. sham operated controls 120 minutes (n=6 laparotomy , 0. 3ml NS at the time correspond to 30 minuteites before reperfusion) , E. ischemia and reperfusion 120 minutes (n=6, 0. 3ml NS 30 minutes before reperfusion and reperfusion for 120 minutes), F. Glutathione pretreatment 120 minutes (n=6, 0. 3ml 20% Glutathione NS solution 30 minites before reperfusion and reperfusion for 120 minutes). Intesine SOD and MDA and serum Tumor necrosis factor—α (TNF—α) were measured . Pathologic methods were used to evaluate thedegree of small intestine injury .Result1. The intestinal SOD value of ischemia and reperfusion group were significantly lower compare with the correspond 60 minutes or 120 minutes sham operated group (P<0. 01, P<0. 01). The intestinal MDA value of ischemia and reperfusion group were significantly higher compare with the correspond 60 minutes or 120 minutes sham operated group (P