Expression And Significance Of P34～(cdc2) And Cyclin B₁ In The Colorectal Carcinoma

Objectives To detect the expression of p34^cdc2, cyclin B₁ in the human colorectal carcinoma and its relationship to the development of colorectal carcinoma.Methods Expression of p34^cdc2 protein, cyclin B₁ and p53 were detected immunohistochemically in 64 humans colorectal carcinomas and 10 normal colon tissues.Results (1)The positive rates of p34^cdc2 and cyclin B₁ expression were 68.75% and 82.81% respectively. The positive staining of p34^cdc2 and cyclin B₁ was mainly located in the cytoplasm of the tumor cells. Weakly to moderately positive expression of p34^cdc2 and cyclin B₁ was mostly seen in the cells in part of the 17 controls. Their expressions were significantly higher in colorectal carcinomas than they were in controls ( P < 0.05 ) . Significant positive correlation was found between the expression of cyclin B₁ and p34^cdc2, the correlation coefficience being 0.586. Statistical significance was marked between lymphatic metastasis and distant metastasis and the expression of cyclin B₁ and p34^cdc2 ( P < 0.05 ) . No statistical significance was found between the differentiated degree and the expression of cyclin B₁ and p34^cdc2. (2)The positive rates of p53 were 73.44% in colorectal carcinomas, and there were no p53 expression in the 10 controls. Significant positive correlations were found between the expression of p34^cdc2 and p53, and no statistical significance was found between cyclin B₁ and p53.Conclusions The overexpression of p34^cdc2 and cyclin B₁ was found in colorectal carcinoma that may be used as a marker in showing the lymphatic metastasis and distant metastasis.