Studies On The Quality Standard Of Huangqi Extract, And The Chemical Constituents Of Cizhuru

This dissertation includes three parts. The first part reviews the progress of the studies on chemical constituents and pharmacological activities in the plants of Astragalus L.. The second part of the article focuses on the study on quality standard of Huangqi extract. The third part elaborates phytochemical investigation of the chemical constituents of Cizhuru. The first part is a review of the research progress of the plants of Astragalus L., which includes their chemical constituents and pharmacology. The second part is about the quality standard of Huangqi extract, and the extract technique of Astragalus Saponins (AS) and Astragalan Polysaccharide (ASP). In chapter one, the methods for the quantitative determination of ASP, AS and astragaloside IV were established. Chapter two analyzed the Chemical constituents of Radix Astragali, the roots of Astagalus mongholicus and A. membranaceus, by high performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESI/MS). Fourteen components were identified as calycosin-7-O-β-D-gluco-pyranoside (1), ononin (2), 9,10-dimethoxyptero-carpan-3-O-β-D-glucopyranoside (3), 2′-hydroxy-3′,4′-dimethoxyptero-carpan-7-O-β-D-glucopyranoside (4), claycosin (5), astragaloside IV (6), astragaloside II (7), isoastragaloside II (8), formaononetin (9), astragaloside I (10), isoastragaloside I (11), sucrose (12), calycosin-7-O-β-D-glucopyranoside-6′′-O-malonate (13) and acetylastragaloside I (14) by direct comparison with the isolated standards, the ESI-MSn, and ultraviolet(UV) spectra.By comparison, we found most of the components were similar except for some minor differences in the two species. Chapter three studied the extraction technology of AS and ASP by orthogonal design. In addition, the technology of isolation and purification of AS by macroporous resin was optimized. Chapter four established a new technology of extraction, isolation and purification of Astragaloside IV, the main active constituent of Huangqi, to improve the yield and optimize the progress. Chapter five generalized the fingerprint of nine samples of Huangqi extract, which were collected from Longxi, Gansu province. At last, chapter six concluded the quality standard of the extract of AS. The third part studied the chemical constituents of Cizhuru, a Chinese traditional medicine obtained from the stem of Sinocalamus affinis (Rendle) McClure. Fourteen known compounds were isolated and identified as: friedelin (1), (24R)-stigmast-4-ene-3-one (2), β-sitosterol (3), (24R)-stigmast-4-ene-3,6-dione (4), 7α-hydroxysitosterol (5), ergost-6,22-dien-3β,5α,8α-triol (6), daucosterol (7), 5,7,4′-trihydroxy-3′,5′-dimethoxyflavone (8), (+)-syringaresinol (9), diethyl sulfone (10), ethyl-p-coumarate (11), p-coumaric acid (12), p-hydroxyphenyl-acetic acid (13) and syringaldehyde (14). All the compounds were isolated from this plant for the first time.