Study On Expression And Their Clinical Significance Of Decorin MRNA,P57～(KIP2) MRNA And TGF-β1 In Lung Cancer

Objective: The genesis of lung cancer was associated with abnormal expression of Decorin mRNA , p57^KIP2 mRNA and TGF-β1. Tissue chip provides a high thoughout tool for studying genes expression and proteins. the aim of this study was to investigate the expressions of Decorin mRNA , p57^KIP2 mRNA and TGF-61, and to analyze their relationships with pathogenesis and clinical charactteristics in lung cancer.Methods: Paraffin embedded tissues from 64 cases of lung cancer and 12 cases of normal lung tissues adjacent to tumor tissues were selected and made to tissue chip, Expressions of Decorin mRNA and p57^KIP2 mRNA were examined by in situ hybridization using labelled digoxigenin probes. Expression of TGF-61 was examined by immunohistochemistry(S-P).All the cases were retrospectively followed up.Results:1.Decorin mRNA and p57^KIP2 mRNA were expressed in cytoderm and cytoplasm, and their positive signals were purple and blue.their expressions followed up.(1) The expression rates of Decorin mRNA were 15.6%(10/64) in tumor tissues and 66.7%(8/12) in adjacent-cancer tissues.The adjacent-cancer tissues showed a higher expression of Decorin mRNA than the tumor tissues(p<0.05). The expression rates of p57^KIP2 mRNA were 32.8% (21/64) in tumor tissues and 25%(3/12) in adjacent-cancer tissues, There was not a significant difference(p>0.05).(2) The expression rate of p57^KIP2 mRNA in squamous cell carcinoma of the lung (LSCC) was 68.4%(13/19), being higher than adenocarcinoma of the lung(LAC) 24% (6/25) ,large cell lung adenocarcinoma(LCLC) 0% (0/4) ,Small cell lung adenocarcinoma (SCLS) 0% (0/9) and alveolar carcinoma 28.6% (2/7), there were significant differences between them. And there were not significant differences in the expression rates of Decorin mRNA between different Pathological grades andclassifications.(3) In lung cancer without lymph node metastasis, The expression of Decorin mRNA44% (8/21) and p57KIP2 mRNA 52% (13/21) increased significantly(p<0.05). In lung cancer with lymph node metastasis, The expression of Decorin mRNA 4.3%(2/43) and p57KIP2 mRNA 17.4% (8/43) decreased significantly.However there was a significant difference(p<0.05).(4) There was not a correlation between Decorin mRNA and p57Kn>2 mRNA,and there were not correlations between Decorin mRNA and p57KIP2 mRNA with clincopathological characteristics of primary lung cancer as well.2. TGF-B1 was expressed in cytoplasm,The positive signal was brown.their expressions followed up.(1) The expression rates of TGF-61 were 84.4% (54/64) in tumor tissues and 83.3% (10/12) in adjacent-cancer tissues, There was not a significant difference between the tumor tissues and adjacent-cancer tissues (p>0.05).(2) The expression rates of TGF-Bl in Small cell lung adenocarcinoma (SCLS) was 33.3%(3/9),being lower than adenocarcinoma of the lung (LAC)91.7%(22/25) ,large cell lung adenocarcinoma (LCLC) 100% (4/4) ,squamous cell carcinoma of the lung ( LSCC) 100% (19/19) and alveolar carcinoma 85.7% ( 6/7), there were significant differences (p<0.05) . Meantime there were not significant differences in the expression rates of TGF-61 between different pathological grades.(3) In lung cancer without lymph node metastasis, The expression of TGF-61 was 71.4% (15/21) .In lung cancer with lymph node metastasis, The expression of TGF-61 was 90.7% (39/43) .However there was not a significant difference for comparing between lung cancer without lymph node metastasis and lung cancer with lymph node metastasis(p>0.05).(4) there were not significant differences between the expression of TGF-61 and clincopathological characteristics(p>0.05).(5) As far as the Spearman relative analysis, there were not significant correlations between the expression of TGF-61, Decorin mRNA and p57KIP2 mRNA (p>0.05).Conclusion : The expression rates of Decorin mRNA in tumour tissues were lower than nomal tissues and the expression rates of Decorin mRNA in lung cancer with lymph node metastasis were lower than In lung cancer without lymph node metastasis. Decorin was a very important component in extracellular matrix, Maybe it partaked the process of tumorigenesis and tumour growth,and there were correlations between Decorin with tumorigenesis,infiltration and metastasis probably. The higher expression rate of Decorin mRNA maybe can keep within limits the tumorigenesis, infiltration and metastasis. pS?11^2 was a cell cycle dependence kinase inhibitor,it inhibited tumorigenesis and tumour growth.lt can be indicated from lower expression rates of p57Kn>2 mRNA in squamous cell carcinoma of the lung and because the malignant degree of squamous cell carcinoma of the lung was lower than other histological category. In lung cancer with lymph node metastasis, the expression of p^^KiP2 mjyvjA decreased significantly and indicated pSl?2 was inhibitor for tumor. The higher expression of pS?131"2 mRNA inhibited tumorigenesis,infiltration and metastasis. There were higher expression rates of TGF-B1 in tumor tissues and adjacent-cancer tissues,and were higher expression rates in lung cancer with lymph node metastasis and lung cancer without lymph node metastasis.As a regulative cytokine, TGF-B1 was expressed extensively in lung cancer and adjacent-cancer tissues.As far as pathologic histology is concerned,there was a relative lower expression of TGF-B1 in small cell lung adenocarcinoma (SCLS) .It indicated that vague mechanism resulted in lower expression of TGF-B1 in formative process of small cell lung adenocarcinoma.But there were relative minor specimens (9 cases) for small cell lung adenocarcinoma in the study,so it could not explain over-mentioned point of view,the study needed more relative investigations to support it.