The Protection And Mechanism Of ATP Pharmacological Postconditioning For Cardiomyocytes With Ischemic Reperfusion

To evaluate the cardioprotective effects offered by adenosine triphosphate (ATP), and discuss it's protective mechanism. In anesthetized open-chest rabbits, the left anterior descending artery(LAD) was occluded for 40 mins and reperfused for 3 hours. In controls(n=16) ,there was no intervention. In preconditioning groups (n=16), the LAD was occluded for 5 mins and reperfused for 10 mins, and repeated this process for three times before the prolonged occluded. In postconditioning groups (n=16), at the start of reperfusion, three cycles of 30-s reperfusion and 30-s LAD reocclusion preceded the 3 hours of reperfusion. In ATP groups (n=16), at the start of reperfusion, ATP(4mg/kg) was injected within 30 mins. The size of NF-kBp65 positive in the control group was significantly larger than that of other groups(P<0.01). Compared with the control groups, the apoptosis rate in the other three groups was lower(P<0.01). Infarct size was significantly smaller in the preconditioning groups, postconditioning groups and ATP groups (P<0.01) compared with control groups. These data suggest that ATP has a good cardioprotective effects for cardiomyocytes with ischemic reperfusion, and this cardioprotective effects is partly due to redusing the cells apoptosis.