| Objective: To investigate the charges of endogenous transforming growth factor β 1(TGF β 1) expression in cardiac muscle of rats following ischemia-reperfusion injury, andthe role of TGF β 1 in cardiac self-repairing and remodeling after injuries due toischemia-reperfusion, and to provide the theoretic basis for the prevention and treatment ofsuch injuries.Methods: The rat model of ischemia-reperfusion injuries was used. Neonatal rats (7-10 days old) were randomly divided into four groups: 1-,7-, 14-day ischemia-reperfusion injury group and normal control group. Expression and distribution of TGF β 1 in cardiomyocytes was examined by qualitative and semiquantitative immunohistochemistry. Masson staining was used in the study of total collagen volume fraction ( CVF). All calculation were performed with SPSS 11.0. Statistical analysis for comparison of data between groups was performed by the one-way analysis of variance. Correlation analysis was performed using pearson correlation coefficient.Results: TGF P 1 gene expression increased markedly at 7-day group (p < 0.01), and peaked at 14-day group (p < 0.01). Compared with the normal control group, CVF increased significantly in 7-day and 14-day ischemia -reperfusion injury groups. Correlation analysis revealed that CVF was positively correlated with TGF β 1 (r=0.574, p< 0.01).Conclusion: TGF β 1 may play an important role in cardiac self-repairing and remodeling after injuries due to ischemia-reperfusion. |
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