| PrefaceThe study of molecular onco - biology shows that the genesis and development of tumour are a complicated biological course of multi - factor, multi -gene and multi - step. The human brain astrocytoma is a common tumour in cranio - cavity. Some oncogene expressed positive in brain astrocytoma, the abnormality of construction and expression of these oncogene is correlative with the genesis and development of tumour. The genesis, development and malignant extent of tumour are probably resulted in by the use of multi - gene. Survivin is an apoptosis suppressor gene of the specificity of tumour, and it belongs to IAP family,which can suppress the apoptosis and play an important role during the course of caryomitosis. As proto - oncogene, survivin is abnormally actived by some carcinogenic agent and results in the genesis of tumour. COX -2 ( Cyclox-ygenase - 2 ) is an important rate - limiting enzyme during the course of the synthesis of prostaglandin via arachidonic acid, and it is closely correlative with the genesis,development of tumour and the apoptosis of tumor cells. Recently, the study shows that the expression of COX - 2 is strong in many kinds of tumors including brain astrocytoma. The expression of COX - 2 is induced because its synthesis is caused by much stimulation, then COX — 2 takes part in many pathological, physiological courses including the inflammation and the apoptosis of tumor cells, thereby promotes the genesis and development of tumour. Although their actions in other tumours have been reported, their coordinate expression and dependablity have been fewly reported in brain astrocytoma. Through the study of the expression of survivin and COX -2 in brain astrocytoma, we may further approach their actions in brain astrocytoma and develop a more effectivepathway for the gene therapy of brain astrocytoma.ObjectiveTo approach the actions of survivin and COX - 2 during the genesis and development in brain astrocytoma and whether they have dependablity each other. By studying their mechanism of molecular biology during the genesis of tumour, we could supply valuable basis of early diagnosis and the prognostic indicator for the clinic and new target gene for the gene therapy of brain astrocytoma.MethodIn our study, there are 14 patients with brain astrocytoma I ,20 patients with brain astrocytoma II , 25 patients with brain astrocytoma H , and 6 patients with brain astrocytoma IV- 10 cases of normal cerebral tissues were selected as negative control. To examine the expression of survivin and COX - 2 via immu-nohistochemistry SP and analysis their interrelationship.Results1. The expressions of survivin and COX - 2 in all samples of normal cerebral tissues are negatively found.2. The positive staining of survivin is mainly located in plasm,and brown -yellow granule could be observed. The positive expressive rates of survivin in astrocytoma grade I , II , I and IV subgroup are 7. 14% ^45% A68% ^83. 33% respectively. It is shown an increased tendency obviously;The rate of higher differentiation group (grade I and II ) is 29.41% (10/34);and the positive expressive rate of lower differentiation group (grade HI and IV ) is 70.97% (22/ 31) , which is obviously higher . There is an significant difference between these two groups(x2=H-20,P<0.01).3. The positive staining of cox -2 is mainly located in plasm , and brown - yellow granule could be observed too . The positive expressive rates of cox -2 in astrocytoma I , II , HI and IVsubgroup are 14. 29% ^35. 00% ^56. 00% ^ 66. 67% respectively. It is shown an increased tendency obviously;The positive expression rate of higher differentiation group ( grade I and II ) is 26.47% (9/34);and the positive expressive rate of lower differentiation group (grade IH and IV ) is 58.06% (18/31) , which is obviously higher . There is an significant difference between these two groups(x2=6.67,P<0.01).4. The positive expressive rate of cox -2 in the group in which survivin expression is positive is 65.63% (21/32);and 18. 18% (6/33) in the group in which survivin expression is negative. There is correlation between them according to spearman correlation analysis: r8 = 0. 522, P < 0.01.ConclusionsThe reactivation of survivin plays a certain important role during the genesis and development of brain astrocytoma. As the malignant extent in brain astrocytoma is more higher, their cell differentiation extent is lower, the positive expressive rate of survivin is more higher.Following with the increase of the malignant extent in brain astrocytoma, the positive expressive rates of COX - 2 show an obviously increased tendency, which indicates COX - 2 gene plays an important role during the genesis and development of brain astrocytoma. Therefore, examining of the expression of COX - 2 gene is helpful to diagnosis of brain astrocytoma.The expressions of COX - 2 and survivin gene in brain astrocytoma are not two isolated events, and they may have certain relation in some link, then they take a joint action during the forming of tumour and result in cell infinite proliferation and missing cell differentiation, thereby to make tumor shows malignant biological behaviour. |
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