| IntroductionBy the development of living level, the phenomena of excessive drink increased greatly. The problem about drink become the social commonality sanitation issue which was concerned by all over the world. 10 -20% drinkers were suffered from alcoholic hepatic injury based on the statistic. The incidence and the death rate of alcohol liver disease (ALD) increased rapidly in these years. β — EP would be released increasdly after alcohol poisoning. Glutathione S -transferase(GST) had perfectly sensitivity and speciality on the reflection of hepatic injury. Malonyldialdehyde( MDA) may reflect the degree of the oxidation stress. P - selectin is an important medium that can introduce the reaction of cells, and play an important role in the inflammation. Puerarin is main portion of the Radix Puerariae, may cure the cardiovascular disease, and gained perfect effect. According to literature, Radix Puerariae has been used for the treatment of alcohol poisoning, but at present there is limited clinical use or basic study a-bout it. So we desire the experiment to study the puerarin. We investigate the affect of the GST , MDA, β- EP , P - selectin and the hepatic tissue pathology by acute alcohol poisoning in rat and the protect effect of the puerarin against the alcohol poisoning to provide experimental or theory basis for its clinical useness.Objective and method1. Animals and drugs24 healthy Wistar rats of both sexes weighting 150 -200g;40% ethanol ig. at dosage of 8. 0 g/kg. d;injection of puerarin;GST, MDA, β - EP and P- selectin reagent.2. Experimental implementFJ - 2008PS -y - radioimmunity recorder, Beckman. DU 640 spectropho-tometer, BIO - RAD Model550 enzymatic remarker.3. Group and methodsRats were divided into 3 groups, each group has 8 rats. Group A (control group) : Saline ip. and ig. GroupB( model group);the same dosage Saline ip. alcohol ig. at dosage of 8g/kg. d. for 5 days to make a model of acute alcohol liver injury. GroupC ( puerarin group) :puerarin ip. at dosage of 200mg/kg. d and 30 minutes later alcohol ig. at same dosage. The blood and liver tissue of each rat were obtained to test the levels of GST, MDA, ￡$ - EP and P - selectin, liver tissue samples were determined pathologically4. Statisticly analysisvAll results were expressd as mean ± standard deviation, conducted by SPSS10. 0 ,T test variance analysis and a P <0. 05 was considered as statistically significant, P<0.01 was considered notablely significant.Result1. commonly conditionThe rats'had blare fur, prompt response, good nutrition in control group;the rats were emaciation with tarnish fur in model group;the rats in puerarin group had better nutrition and rapidly response than those in model group.2. The effects 'of alcohol poisoning on the levels of GST, MDA , (B - EP and P - selectin in rats' blood and the effects of puerarin.The levels of GST , MDA, f$ - EP and P - selectin were significantly increased in model group as compared with that in control group (GST^p -EP;P < 0. 01, P - selectin, MDA: P < 0. 05 );and decreased in puerarin group than that in model group ( P <0.05).3. The effects of alcohol poisoning on the levels of GST and MDA in rats' hepatic homogenates and the effects of puerarinThe levels of GST, MDA in the rats'hepatic homogenates were increasedsignificantly in model group than that in control group (MDA P <0. 05;GST P < 0. 01);and that in puerarin group were much lower than in model group(P <0. 05) o4. The effect of alcohol on the rats' hepatic pathology and the affection of the pretreatment with puerarin4. 1 The pathological changes under optic microscopeThe cells and structure of hepatic tissue were nearly normal in control group. The structure of hepatic tissue was unclear, the cells were in a mass, the hepatic sinus freezed, and there were fatty - , hydro, balloon degeneration or local necrosis in model group. The hepatic tissue structure and the cells were much better in puerarin group than that in model group.4. 2 The pathological changes under electro - mictroscope.The cells and structure of hepatic tissue were nearly normal in control group, there ware fatty degeneration, Endoplasmic Reticulum expand , hepatin decreased, mit augment, abnormality, cells organ in the plasm decrease either in model group. The hepatic tissue and cells much better in puerarin group than that in model group.DiscussionTaken excessive alcohol in a short periods may lead to acute alcoholic liver injury. Our experiment showed that acute alcohol poisoning increased the levels of GST and MDA in rats'serm or homogenates, the injury of hepatic tissue with fatty -1 , hydro or balloon degeneration, local necrosis there. And the mechanism maybe alcohol induce free radical generation, which lead to lipid peroxida-tion. From our experiment, by pretreatment with puerarin, the pathology deviation and the levels of GST, MDA decrease than in model group. Those suggested that puerarin may improve the function of alcohol poisoning rats, which associated with its' effect of antioxidation, and alleviation lipid peroxidation of hepatic cells. The levels of (3 - EP increased significantly in model group and decreased in puerarin group than in model group. That suggested the hepatic injury may associated with the function of p - EP inhibiting the activation of hepatic orni-thine decarboxylase and Puerarin play a protect role by preventing (3 - EP releasing. P - selectin induced the reaction of cells and associated with immunity, inflammation. Our study showed that acute alcohol poisoning increased the level of P - selectin, Puerarin inhibit its express and liberation, but the mechanism is unclear.ConclusionsAcute alcohol poisoning induced acute hepatic injury , increase the levels of GST and MDA, damaged the hepatic pathology. Puerarin had protective effects on the hepatic damage caused by acute alcohol poisoning for its effects on anti - oxygen free radicals, resisting lipid peroxidation injury, inhibiting of (3 -endorphin and P - selectin releasing. It could be used in the treatment of alcohol poisoning. |
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