Changes Of Free Amino Acids In Rats During Simulated Sea Sickness And Post-adaptation

Objective To observe the changes of free amino acids (FAAs) in plasma and tissue of rats during simulated sea sickness and post-adaptation. Method: Pica or kaolin consumption was used as a indicator to judge the development of sea sickness and adaptation when SD rats were stimulated by Crampton sea sickness simulator. FAAs concentrations in the plasma and of tissue rats were determined by high performance liquid chromatography (HPLC) after simulated sea sickness and post adaptation. Result: After simulated sea sickness stimulation for 1 day. the levels of Cys and Ile were increased, and Gly and Pro decreased significantly in the plasma. The increase of BCAA/AAA ratio was also found. In brains, the contents of Ala, Cys+Met, Tyr. His, and total amino acids were remarkably decreased. In Liver, the contents of Ser, Val, Ile, Leu, Phe, Lys remarkably increased. The content of His and the ratio of AAA/BCAA decreased. In kidney, the level of Ser, Ala, Phe decreased. After simulated sea sickness stimulation for 21 days. the ratio of Glu/GABA increased in brains. The content of EAA and the ratio of EAA/TAA decreased significantly in kidney. Conclusion: The levels of amino acids. especially those related to neurotransmitter synthesis. in plasma and tissues changed significantly during simulated sea sickness in the rats.