| Objective: The effect to induce NSCs proliferation and differentiation in the adult rat SGZ, SVZ and cortex was investigated by NMDA receptor antagonist administration following focal ischemic insults produced by middle cerebral artery occlusion (MCAO).Methods: Forty SD rats are assinged two groups: experiment group (I), control group (II). All rats were subjected to 2h of MACO. Rats of group (I) were intraperitoneal the NMDA receptor antagonist MK-801 and rats of group(II) were intraperitoneal saline. The rate of BrdU postive cells and Nestin postive cells were compared in SGZ, SVZ and infract contex for 3, 7,11 and 18 days.Results: Group (II): BrdU postive cells and increased in SVZ 3 days after reperfusion, reached slight peak after 7 day in SGZ, decreased after 11 days and scarely detected after 18 days, especially in the infarct cortex. But Gorup (I): treatment by MK-801, BrdU positive cells significally increased than group (II )after 3, 7, 11 and 18 days in SGZ and SVZ, the cells number of BrdU were respective 44 + 7 unit /mm2, 451 ±55 unit/mm2, 320±32 unit/mm2, 138+28 unit/mm2 and 50 + 8 unit/mm2, 350+39 unit /mm2, 270+28 unit /mm2, 110 + 28 unit /mm2 (P<0.01). Compared two Group, the number of BrdU cells in the infarct cortex, it was not different for 3 day (P>0.05), but Gorup (I) significally increased for 7, 11 and 18day, were respective 267 ±38 unit /mm2, 190 ±26 unit /mm2, 82 ±7 unit /mm2 (P<0.01). The number of Nestin cells were.scarely come forth in two Groups (P>0.05), but compared two Groups, Gorup(I) significally increased for 11 and 18 day in SGZ; SVZ and infarct cortex (P<0.01).The proliferation is found increasing long-lasting after 18 days. Expecially, BrdU Nestin postive cells singnifically expressed near infract contex.Conclusion: NMDA receptor antagonist treatment serves as a valuable tool to activate NSCs proliferation and differentiation in cerebral after infract. |
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