Basic Pharmaceutical Researches On Radix Isatidis And Rhizoma Et Radix Baphicacanthis Cusiae

Objective: To compare the differences between Radix Isatidis and Rhizoma et Radix Baphicacanthis Cusiae. Contents: Studies on the antimicrobial activities, extraction method and quality control, chemical constituents of Radix Isatidis and Rhizoma et Radix Baphicacanthis Cusiae. Methods: 1. The antibacterial activities of different chemical fractions from these two materials against S. aureus, E. Coli, B. subilis and C.albicans were tested with disc agar diffusion method and MIC of the most active part were decided by agar dilution method, respectively. 2. These two materials were extracted with traditional isolation method and systematic solvents isolation method, respectively. Extraction effects were determined by antimicrobial activity tests. Optimum extract method and quality control were discussed. 3. Radix Isatidis and Rhizoma et Radix Baphicacanthis Cusiae were percolated with solvents and the components of active fraction were isolated and purified. The structures were identified on the basis of spectral data. Results: 1. Bacteriostasis of the both materials were broad-spectrum. The active components of Radix Isatidis are in moderate to high polar while the active components of Rhizoma et Radix Baphicacanthis Cusiae are separate. The 8^th part in EtOAc-MeOH fraction was the most active part to both materials, and their MIC of Rhizoma et Radix Baphicacanthis Cusiae and Radix Isatidis to 5. aureus were 0.125mg/ml and 0.25 mg/ml while to E.Coli were 0.25mg/ml and 1mg/ml. 2. The extraction effects of traditional isolation method were worse than that of systematicsolvents isolation method for the latter can concentrate the active components thus improve the efficacy. The quantity of a single active component shouldn't be regarded as unique quality control. 3. Twelve compounds were isolated from Rhizoma et Radix Baphicacanthis Cusiae, and six of them were first isolated from this material and the plants of genus of Baphicacanthus . Moreover, flavonoids were isolated for the first time from Rhizoma et Radix Baphicacanthis Cusiae. 4. Eight compounds were isolated from Radix Isatidis, and four of them were first isolated from this material and the genus, of Isatis L. Conclusions: Radix Isatidis and Rhizoma et Radix Baphicacanthis Cusiae are different. It's necessary for deeply continuing the researches in order to make good uses of each of them.