Expression Of EG-VEGF In Nomal Human Ovarian Cycle

Objective: To investigate the expression and localization of EG-VEGF mRNA in the normal ovarian cycle. And to compare the expression of EG-VEGF mRNA and VEGF mRNA and the relationship in the physiological ovarian angiogenesis. Method: 23 childbearing aged women were classified into follicular phase (n=11) and luteal phase (n=12) according to their last menstrual date and levels of serum E2, P, who underwent surgery because of non-ovary disease during Oct. 2003- Mar. 2004. Quantitative RT-PCR and in site hybridization were used to determine changes of EG-VEGF mRNA and VEGF mRNA expression in the ovarian cycle. In site hybridization was also used to localize expressive sites and diversity of EG-VEGF mRNA and VEGF mRNA. Meanwhile, the relatioship between EG-VEGF mRNA and VEGF mRNA expression was analyzed. Result: The expression of EG-VEGF mRNA was much higher than VEGF mRNA in both follicular and luteal phase (P<0.05) by Quantitative RT-PCR detection; The expression of VEGF mRNA in the follicular phase was lower than that in the luteal phase (P<0.05), but there was no significant difference of EG-VEGF mRNA expression between follicular and luteal phase. In site hybridization revealed that, higher EG-VEGF mRNA expression appeared in primordial and primary follicles, then declined with the development of follicle; whereas VEGF mRNA expression was weak in primordial and primary follicle, itincreased as secondary follicles matured. During the luteal phase, the expression of VEGF mRNA was the strongest in early corpus luteum, then declined as luteal mature and undetectable in the late luteal phase; However, EG-VEGF mRNA expression started to increase from early- and mid-luteal phase, and arrived the highest in the late luteal phase. Conclusion: There exists spatical and temporal difference between the expression of EG-VEGF mRNA and VEGF mRNA in follicular and luteal phase of nomal ovarian cycle, indicate that they have different action and different mechanism in physiological ovarian angiogenesis.